Figure 1

Tight regulation of the CcpA expression level in B. subtilis strain MP902 (P tet - ccpA , P xyl - tetR ). Lane 1, wild type strain 168; lanes 2 – 11, MP902 grown in presence of 0.2 % xylose and increasing concentration of anhydrotetracycline (ATc): 0.1, 0.2, 04, 0.7, 1, 2, 4, 8, 10 and 20 nM, respectively; lane 12, 200 ng of purified CcpA. The representative graph of three reproducible experiments is shown.