Target validation. (a) Separation of 5' RACE products of target genes for cca-miR-160 (left gel), and cca-miR-393, 397, 398 (right gel, from left to right). Asterisks indicate cleaved fragments. (b) Partial sequence of TIR1 homolog from globe artichoke (acc. No. JN382008). 21 nucleotide register phasing of sense (red) siRNAs were observed following 5' RACE analysis. Canonical and observed miR393 cleavage sites are indicated by green arrows.