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Table 6 Validation of microarray results and expression of genes of interest in relation to the factor 'Lipid level'

From: Hepatic transcriptome analysis of inter-family variability in flesh n-3 long-chain polyunsaturated fatty acid content in Atlantic salmon

Gene Low n-3 LC-PUFA HL/LL High n-3 LC-PUFA HH/LH
  Microarray RT-qPCR Microarray RT-qPCR
LC-PUFA biosynthesis
Δ5fad   −1.03   1.35
Δ6fad   1.04   −1.32
elovl2 −1.28 to −1.41 −1.51 −1.82 to −1.97 −1.62
Cholesterol biosynthesis   
ipi   −3.95   1.31
mev   −1.40   1.14
7dchr   −1.01   1.09
srebp2   −2.09   1.29
Lipid transport and lipoprotein metabolism  
lrp1   −1.82   −1.08
apoA4a   −1.37   −0.95
apoA4b   −2.43   −1.80
lpl   −1.18   −1.05
Immune response
cfh −1.33 to −1.56 −1.24 −1.56 to −1.69 −1.31
trim25 −5.64 −2.09 −4.34 −2.33
lect2 3.16 1.23 4.86 4.84
  1. Values represent the expression ratios between high lipid / low lipid, for fish containing either low or high n-3 LC-PUFA levels in their flesh, obtained by microarray analysis or RT-qPCR. Expression ratios in bold were significant by REST2008 analysis of RT-qPCR results.
  2. Delta5 and 6 fatty acyl desaturases (Δ5fad and Δ6fad); fatty acyl elongase (elovl2); isopentenyl-diphosphate isomerase (ipi); mevalonate kinase (mev); 7-dehydrocholesterol reductase (7dchr); sterol regulatory element-binding protein 2 (srebp2); low density lipoprotein receptor-related protein 1 (lrp1); apolipoprotein A-IV (apoa4a and apoa4b); lipoprotein lipase (lpl); complement factor H precursor (cfh); tripartite motif-containing protein 25 (trim25); leukocyte cell-derived chemotaxin 2 precursor (lect2).