The expression of four miRNA-target pairs during five developmental stages in common carp. Expression levels of miRNAs and their predicted targets were detected by RT-qPCR at five developmental stages (X axis). Y axis shows the expression levels of miRNA and its target. The expression of each miRNA was normalized to U6 and then transformed to a log 2 scale. The expression of each target gene was relative to beta-actin and also transformed to a log 2 scale. The value of r is the correlation coefficient. All four miRNA-target pairs showed significantly reciprocal expression patterns (p ≤ 0.05). Developmental stages are specified in Figure 4 legend.