Table 4 Targets of conserved B. napus miRNAs
miRNA | Target EST | Category | Cleavge site | Reads mapping to the expected cleavage site | Percentage of expected reads to total reads mapped to the full length of EST (%) | Target site location | Target annotation |
|---|---|---|---|---|---|---|---|
Bna-miR156a | EL625881 | 4 | 455 | 5 | 19 | 3′UTR | A. thaliana SPL3 transcription factor |
|  | EV190718 | 2 | 681 | 3 | 38 | 3′UTR | A. thaliana SPL10 transcription factor |
|  | EV184491 | 1 | 747 | 2 | 100 | 3′UTR | A. thaliana O-fucosyltransferase family protein |
Bna-miR156c | EV190718 | 2 | 682 | 3 | 38 | 3′UTR | A. thaliana SPL10 transcription factor |
Bna-miR159 | EV087133 | 4 | 439 | 2 | 20 | ORF | A. thaliana MYB65 |
| Â | EV223870 | 4 | 279 | 5 | 25 | ORF | metallo-beta-lactamase family protein |
| Â | EV136053 | 4 | 685 | 3 | 21 | ORF | pyruvate, phosphate dikinase (PPDK) |
Bna-miR160a | EV006535 | 0 | 256 | 2 | 50 | ORF | auxin response factor |
Bna-miR164b | ES906443 | 4 | 68 | 3 | 20 | ORF | NAC domain-containing protein |
Bna-miR167b | ES962471 | 4 | 264 | 3 | 25 | ORF | Auxin response factor 8 |
Bna-miR167c | EV208388 | 4 | 657 | 3 | 27 | ORF | B. rapa IAA-amino acid hydrolase 3 |
Bna-miR169a | EE543166 | 0 | 247 | 3 | 50 | 3′UTR | A. thaliana NF-YA3 |
|  | CN729971 | 4 | 406 | 3 | 17 | 3′UTR | A. thaliana NF-YA5 |
Bna-miR169e | ES980547 | 2 | 459 | 5 | 36 | 3′UTR | A. thaliana NF-YA3 |
|  | EE543166 | 0 | 247 | 3 | 50 | 3′UTR | A. thaliana NF-YA3 |
|  | CN729971 | 4 | 406 | 4 | 22 | 3′UTR | A. thaliana NF-YA5 |
Bna-miR169l | ES980547 | 2 | 459 | 5 | 36 | 3′UTR | A. thaliana NF-YA3 |
|  | ES959135 | 4 | 352 | 3 | 27 | 3′UTR | B. napus clone bncbf-b2 |
|  | EE543166 | 0 | 247 | 3 | 50 | 3′UTR | A. thaliana NF-YA3 |
|  | CN729971 | 4 | 406 | 4 | 22 | 3′UTR | A. thaliana NF-YA5 |
Bna-miR171b | ES907976 | 3 | 609 | 3 | 33 | ORF | B. napus SCL1 |
| Â | ES902868 | 3 | 673 | 4 | 34 | ORF | A. thaliana SCL6-IV |
Bna-miR171f | ES902868 | 4 | 676 | 3 | 21 | ORF | A. thaliana SCL6-IV |
| Â | ES907976 | 4 | 612 | 4 | 25 | ORF | B. napus SCL1 |
Bna-miR171g | ES902868 | 4 | 676 | 3 | 23 | ORF | A. thaliana SCL6-IV |
| Â | ES907976 | 4 | 612 | 2 | 18 | ORF | B. napus SCL1 |
Bna-miR172f | FG568924 | 4 | 488 | 2 | 20 | ORF | B. napus APETALA2 |
| Â | EV197066 | 0 | 677 | 6 | 50 | ORF | A. thaliana AP2-like protein (At2g28550) |
| Â | DY025256 | 4 | 609 | 5 | 24 | ORF | A. thaliana AP2-like protein (SMZ) |
Bna-miR390a | EV220086 | 0 | 433 | 4 | 50 | ORF | cytochrome P450 family 78, subfamily A |
Bna-miR390d | EV220086 | 0 | 433 | 4 | 50 | ORF | cytochrome P450 family 78, subfamily A |
Bna-miR393 | EL628991 | 2 | 246 | 10 | 17 | ORF | A. thaliana auxin signaling F-box 3 |
| Â | EV176346 | 0 | 564 | 6 | 50 | ORF | A. thaliana auxin signaling F-box 3 |
Bna-miR396a | ES923674 | 4 | 571 | 1 | 20 | ORF | A. thaliana bHLH74 transcription factor |
Bna-miR397b | ES906654 | 4 | 736 | 3 | 20 | ORF | A. thaliana laccase-4 (IRX12) |
| Â | EE460611 | 4 | 445 | 5 | 25 | ORF | A. thaliana 60S ribosomal protein L15 |
Bna-miR399 | EV157460 | 4 | 268 | 3 | 23 | 5′UTR | A. lyrata PHO2/UBC24 |
| Â | CX281881 | 4 | 581 | 5 | 23 | ORF | B. napus genes for ITS1, ITS2 |
Bna-miR408a | EE417826 | 4 | 457 | 2 | 20 | ORF | A. thaliana peptide chain release factor 1 |
Bna-miR408b | EV075738 | 4 | 63 | 2 | 22 | 5′UTR | A. thaliana plantacyanin |
Bna-miR824 | EV112524 | 0 | 319 | 4 | 50 | ORF | A. thaliana MADS-box protein AGL16 |
miR1140b | EV217683 | 0 | 473 | 3 | 50 | ORF | T. aestivum mRNA for glycosyltransferase |
| Â | ES912747 | 0 | 119 | 1 | 50 | ORF | A. thaliana two-component response regulator ARR8 (RR3) |
Bna-miR2111b | EV221566 | 0 | 337 | 3 | 50 | ORF | A. thaliana F-box/kelch-repeat protein |