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Table 4 Targets of conserved B. napus miRNAs

From: Identification of miRNAs and their targets from Brassica napus by high-throughput sequencing and degradome analysis

miRNA Target EST Category Cleavge site Reads mapping to the expected cleavage site Percentage of expected reads to total reads mapped to the full length of EST (%) Target site location Target annotation
Bna-miR156a EL625881 4 455 5 19 3UTR A. thaliana SPL3 transcription factor
  EV190718 2 681 3 38 3UTR A. thaliana SPL10 transcription factor
  EV184491 1 747 2 100 3UTR A. thaliana O-fucosyltransferase family protein
Bna-miR156c EV190718 2 682 3 38 3UTR A. thaliana SPL10 transcription factor
Bna-miR159 EV087133 4 439 2 20 ORF A. thaliana MYB65
  EV223870 4 279 5 25 ORF metallo-beta-lactamase family protein
  EV136053 4 685 3 21 ORF pyruvate, phosphate dikinase (PPDK)
Bna-miR160a EV006535 0 256 2 50 ORF auxin response factor
Bna-miR164b ES906443 4 68 3 20 ORF NAC domain-containing protein
Bna-miR167b ES962471 4 264 3 25 ORF Auxin response factor 8
Bna-miR167c EV208388 4 657 3 27 ORF B. rapa IAA-amino acid hydrolase 3
Bna-miR169a EE543166 0 247 3 50 3UTR A. thaliana NF-YA3
  CN729971 4 406 3 17 3UTR A. thaliana NF-YA5
Bna-miR169e ES980547 2 459 5 36 3UTR A. thaliana NF-YA3
  EE543166 0 247 3 50 3′UTR A. thaliana NF-YA3
  CN729971 4 406 4 22 3′UTR A. thaliana NF-YA5
Bna-miR169l ES980547 2 459 5 36 3′UTR A. thaliana NF-YA3
  ES959135 4 352 3 27 3UTR B. napus clone bncbf-b2
  EE543166 0 247 3 50 3UTR A. thaliana NF-YA3
  CN729971 4 406 4 22 3UTR A. thaliana NF-YA5
Bna-miR171b ES907976 3 609 3 33 ORF B. napus SCL1
  ES902868 3 673 4 34 ORF A. thaliana SCL6-IV
Bna-miR171f ES902868 4 676 3 21 ORF A. thaliana SCL6-IV
  ES907976 4 612 4 25 ORF B. napus SCL1
Bna-miR171g ES902868 4 676 3 23 ORF A. thaliana SCL6-IV
  ES907976 4 612 2 18 ORF B. napus SCL1
Bna-miR172f FG568924 4 488 2 20 ORF B. napus APETALA2
  EV197066 0 677 6 50 ORF A. thaliana AP2-like protein (At2g28550)
  DY025256 4 609 5 24 ORF A. thaliana AP2-like protein (SMZ)
Bna-miR390a EV220086 0 433 4 50 ORF cytochrome P450 family 78, subfamily A
Bna-miR390d EV220086 0 433 4 50 ORF cytochrome P450 family 78, subfamily A
Bna-miR393 EL628991 2 246 10 17 ORF A. thaliana auxin signaling F-box 3
  EV176346 0 564 6 50 ORF A. thaliana auxin signaling F-box 3
Bna-miR396a ES923674 4 571 1 20 ORF A. thaliana bHLH74 transcription factor
Bna-miR397b ES906654 4 736 3 20 ORF A. thaliana laccase-4 (IRX12)
  EE460611 4 445 5 25 ORF A. thaliana 60S ribosomal protein L15
Bna-miR399 EV157460 4 268 3 23 5UTR A. lyrata PHO2/UBC24
  CX281881 4 581 5 23 ORF B. napus genes for ITS1, ITS2
Bna-miR408a EE417826 4 457 2 20 ORF A. thaliana peptide chain release factor 1
Bna-miR408b EV075738 4 63 2 22 5UTR A. thaliana plantacyanin
Bna-miR824 EV112524 0 319 4 50 ORF A. thaliana MADS-box protein AGL16
miR1140b EV217683 0 473 3 50 ORF T. aestivum mRNA for glycosyltransferase
  ES912747 0 119 1 50 ORF A. thaliana two-component response regulator ARR8 (RR3)
Bna-miR2111b EV221566 0 337 3 50 ORF A. thaliana F-box/kelch-repeat protein