Table 5 Targets of candidate novel B. napus miRNAs
miRNA | Target EST | Category | Cleavage site | Reads mapping to the expected cleavage site | Percentage of expected reads to total reads mapped to the full length of EST (%) | Target site location | Target annotation |
|---|---|---|---|---|---|---|---|
Bna-miRC2 | EV142354 | 1 | 347 | 6 | 30 | ORF | A. lyrata PPR-containing protein |
Bna-miRC5-2 | EV077017 | 0 | 326 | 4 | 50 | ORF | A. lyrata exostosin family protein |
Bna-miRC5-5Bna-miRC8 | EV154449 FG574835 | 2 4 | 296 119 | 3 2 | 27 20 | ORF 5′UTR | A. thaliana alpha-tubulin 6 A. thaliana uncharacterized protein |
Bna-miRC9 | EV006535 | 0 | 256 | 2 | 50 | ORF | A. thaliana auxin response factor 17 |
Bna-miRC13 | EV022057 | 1 | 105 | 3 | 30 | ORF | A. thaliana protein PIR |
Bna-miRC15-1 | EV191962 | 0 | 132 | 5 | 50 | 5′UTR | A. thaliana ferrochelatase 1 |
Bna-miRC15-2 | EV054423 | 2 | 615 | 3 | 33 | ORF | A. lyrata ferrochelatase 1 |
Bna-miRC16 | GR445128 | 3 | 416 | 4 | 27 | ORF | B.juncea chlorophyll a/b-binding protein |
Bna-miRC17a-1 | CD818234 | 1 | 647 | 5 | 36 | ORF | A. thaliana cinnamyl alcohol dehydrogenase |
Bna-miRC18 | GT074945 | 2 | 341 | 12 | 30 | ORF | B.napus GF14 omega |
Bna-miRC20-1 | GR442870 | 0 | 578 | 3 | 50 | ORF | A. thaliana histone H2B-like protein |
| Â | ES987065 | 0 | 39 | 3 | 50 | ORF | B.rapa photosystem II center W-like protein |
Bna-miRC21 | GT079646 | 2 | 65 | 3 | 33 | 5′UTR | A. thaliana photosystem I subunit XI |
Bna-miRC22a-1 | EV044066 | 3 | 505 | 4 | 27 | ORF | A. thaliana OST3/OST6 family protein |
Bna-miRC26 | EV077764 | 0 | 593 | 5 | 50 | ORF | A.thaliana uncharacterized protein (AT3G51610) |
Bna-miRC30 | EV025081 | 2 | 329 | 4 | 36 | ORF | Glycosyl hydrolase family protein |
| Â | CX189212 | 3 | 269 | 3 | 27 | ORF | Glycosyl hydrolase family protein |
Bna-miRC47 | ES992448 | 0 | 517 | 6 | 50 | ORF | A. thaliana prenylcysteine oxidase (FCLY) |