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Table 3 Identification of genes putatively involved in the biosynthesis of various vitamins and siroheme

From: Genetic diversity within the genus Francisella as revealed by comparative analyses of the genomes of two North American isolates from environmental sources

Function Locus tag (Protein) Annotation Closest homolog outsideFrancisella locus tag, protein, identity,E-value
Thiamine biosynthesis   
Fphi_0086 (251 aa) Thiazole biosynthesis adenylyltransferase (ThiF) Mrub_1727, 266 aa, 38%, 7e-41
Fphi_0087 (485 aa) Fused protein Phosphomethylpyrimidine kinase (ThiD)/Thiamine-phosphate pyrophosphorylase (ThiE) lpg1568, 495 aa, 35%, 1e-72
Fphi_0088 (259 aa) Thiazole synthase (ThiG) lpg1567, 263 aa, 58%, 2e-86
Fphi_1923 (66 aa) Thiamine biosynthesis protein (ThiS) IL0768, 66 aa, 32%, 1e-04
Fphi_0089 (350 aa) Thiamine biosynthesis oxidoreductase (ThiO) Kkor_0127, 351 aa, 34%, 3e-47
Fphi_0090 (592 aa) Thiamine biosynthesis protein (ThiC) CV_0235, 632 aa, 72%, 0.0
Riboflavin biosynthesis   
Fphi_0395 (306 aa) Riboflavin kinase (RibF) D11S_1924, 308 aa, 47%, 5e-69
Fphi_0713 (356 aa) Riboflavin biosynthesis protein (RibD) Acear_1431, 371 aa, 44%, 5e-79
Fphi_0714 (201 aa) Riboflavin synthase, alpha subunit (RibC/RibE) CLD_1677, 228 aa, 48%, 5e-41
Fphi_0715 (403 aa) GTP cyclohydrolase II (RibA) DEFDS_1098, 405 aa, 49%, 5e-104
Fphi_0716 (147 aa) 6,7-dimethyl-8-ribityllumazine synthase (RibH) lpg1180, 155 aa, 56%, 3e-39
Folate biosynthesis   
Fphi_0420 (282 aa) Methenyltetrahydrofolate cyclohydrolase (FolD) SULAZ_0428, 284 aa, 60%, 3e-87
Fphi_0547 (394 aa) Dihydrofolate synthase (FolC) Ssed_1653, 421 aa, 34%, 2e-45
Fphi_0600 (165 aa) Dihydrofolate reductase (FolA) BMD_4044, 161 aa, 54%, 7e-41
Fphi_1791 (184 aa) Para-aminobenzoate synthase, amidotransferase component Slin_0655, 205 aa, 38%, 4e-37
Fphi_1792 (587 aa) Para-aminobenzoate synthase, aminase component Csal_2692, 629 aa, 37%, 3e-99
Fphi_1794 (117 aa) Dihydroneopterin aldolase (FolB) WPa_0696, 125 aa, 32%, 1e-09
Fphi_1795 (421 aa) Dihydropteroate synthase (FolKP) RBE_0032, 438 aa, 35%, 4e-48
Biotin biosynthesis   
Fphi_1798 (428 aa) Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (BioA) CBU_1008, 442 aa, 51%, 4e-121
Fphi_1799 (313 aa) Biotin synthase (BioB) CBU_1007, 321 aa, 61%, 7e-103
Fphi_1800 (372 aa) 8-amino-7-oxononanoate synthase (BioF) Sde_3138, 397 aa, 38%, 6e-60
Fphi_1801 (244 aa) Biotin biosynthesis protein (BioC) CBU_1004, 248 aa, 28%, 2e-10
Fphi_1803 (226 aa) Dethiobiotin synthase (BioD) Sde_3135, 230 aa, 38%, 5e-37
Siroheme biosynthesis   
Fphi_0284 (323 aa) Porphobilinogen synthase (HemB) Amet_0062, 324 aa, 60%, 1e-110
Fphi_0603 (469 aa) Glutamyl-tRNA synthetase (GltX) Fbal_2571, 470 aa, 60%, 2e-163
Fphi_0691 (300 aa) Porphobilinogen deaminase (HemC) APL_1010, 309 aa, 56%, 4e-86
Fphi_0945 (344 aa) Uroporphyrinogen decarboxylase (HemE) Rmag_1026, 347 aa, 50%, 1e-97
Fphi_1071 (414 aa) Glutamyl-tRNA reductase (HemA) PSPA7_5315, 422 aa, 35%, 2e-70
Fphi_1313 (252 aa) Uroporphyrinogen-III synthase (HemD) PSM_A0104, 613 aa, 31%, 1e-08
Fphi_1400 (400 aa) Protoporphyrinogen oxidase (HemY/HemG) Cphamn1_2137, 396 aa, 34%, 2e-58
Fphi_1812 (433 aa) Glutamate-1-semialdehyde aminotransferase (HemL) APJL_1583, 426 aa, 61%, 2e-151
Fphi_1842 (308 aa) Coproporphyrinogen III oxidase (HemF) Tcr_0017, 323 aa, 59%, 2e-106
Fphi_1900 (338 aa) Ferrochelatase, protoheme ferro-lyase (HemH) SMc04019, 342 aa, 50%, 7e-92