Figure 1

Genomic distribution of H3K9ac and H3K14ac in mES cells. (A) Distribution of H3K9ac and H3K14ac peaks over the promoters (2000 bp upstream of TSS,), coding exons, introns and distal intergenic regions. Many of the H3K9 and H3K14 acetylation peaks are at distal intergenic regions. (B) Dot plot representation of genome-wide co-localization analysis of the H3K9ac and H3K14ac modifications over the 15595 combined promoter list of H3K9 and H3K14ac suggests a strong correlation between these two modifications at promoters. (C) Average input normalized profile of 15595 combined promoter list of H3K9 and H3K14ac around the transcription starts sites shows bimodal distribution. (D) Average input normalized whole gene profiles for H3K9ac and H3K14ac modifications over 15595 combined promoter list of H3K9 and H3K14ac. (E) Sequential ChIP–qPCR quantification for co-occupancy of H3K9ac (primary ChIP) and H3K14ac (secondary ChIP) at randomly selected H3K9 and H3K14 acetylated loci suggest that these loci are co-marked with H3K9 as well H3K14 acetylation. Enrichment after first ChIP using H3K9ac followed by re-ChIP with no antibody was used as a control. Primer sequences used in ChIP-qPCR is provided in Additional file4: Table S1. Error bars represent the standard deviation for three technical replicates.