Table 1 Characteristics of 50 EST-SSR primers developed in L. luteus. Shown for each primer pair are the library specificity, repeat motif, forward and reverse sequence, allele range size (bp), number of alleles, amplification in other Lupin species, and annotation

l1l2itg33000

L1

(ACA)7

CACGTCAGTCCTTGCACCTA

GCACAGCAACAACAACACAA

129-132

2

L.hispanicus

l1l2itg51784

L1

(TA)8

CATCCTTCAAAAACCATTTCAA

AATGTTGATGAACGCGTGTG

274-280

3

l1l2itg52347

L1

(AT)8

CTCATGTTTCTTGGGTGGAAA

CAATCATGTCTAAACCGGGAA

209-215

4

l1l2itg50343

L1

(AT)10

ATATTAGCGGCCATGCTGTT

TGTTCATGTTGGTTGCAAGA

235-239

3

l1l2itg20858

L1

(AAC)12

ACCCCACTTCTCCCAACTCT

TCCATGAATGAAATGGGGTT

229-238

3

L.hispanicus

Pollen-specific protein SF3

l1l2itg20038

L1

(TA)9

TTCAGAAACAAAGGGGTTGC

TCCAGAAATTCTTCTACATCCCA

179-183

3

l1l2itg52625

L1

(TCA)12

CTGGTCTTCTGTCGACTCCA

GACCAAGAAGTCAAGCTCGG

109-124

4

l1l2itg37631

L1

(CT)12

TAAAGTGCCACCAACAAGCA

TTGTGTTGGTTGTGTGTAGAGAGA

133-155

6

l1l2itg27097

L1

(AAT)7

TTCAACTACCGGTTGAACCAC

GCCCAGAATTAGGGTGCTTT

206-209

2

l1l2itg22424

L1

(GAA)7

AAACGACCAACCGCATAAAG

GATGCGTGAAACTGCAAAGA

240-249

3

L.hispanicus

N-acetylglutamate synthase

l1l2itg29703

L1

(GA)8

ACCTTTGCGCCAAGATACAC

ATTGTGACGGTTTCACTCCC

213-219

4

l1l2itg28437

L1

(TA)9

GGGCACATTTGACTCTTTCG

TCCGTGCAATGTCAATATCAA

260-268

4

L.hispanicus

l1l2itg36804

L1

(ATA)12

CACATGAGAAGCAGCAATGAA

ATGCGGTGGAGTGGAAGTAA

254-260

2

L.hispanicus

l1l2itg21177

L1, L2

(CAT)8

CCTTGAGGCCAATAAATGGA

TTAAGGAAGCTAGGGCCACA

217-226

3

L.hispanicus

Delta-8 sphingolipid desaturase

l1l2itg39645

L1

(ATT)10

AATCATGGCCTTTTTGCTTG

CGTCTTGCTCTGGTTCTTCC

148-169

5

l1l2itg35309

L1

(TA)8

TTCATGGCAAGAAAAACATCT

AATCATCCATGCCATTTAACA

271-281

4

l1l2itg56943

L1

(GA)8

GAGGCCCAAAAACAGAAACA

CCATTTGCGTTCGGTTCTAT

270-272

2

L.hispanicus, L.mutabilis

l1l2itg31693

L1

(TAT)8

AGGGGCAAAGCTCAAAGACT

CATTCACATTTTATCCTCATTGACTC

196-217

4

L.hispanicus

l1l2itg10347

L1

(AT)8

TGTGGTAAATGCAGGCTCAG

ATGCAACGGGAACCATAGTC

184-186

2

L.hispanicus

l1l2itg14618

L1

(CAT)7

TTCCTCATCTCCCACACCTC

AGCTTCTGCTTGTAATCGGC

237-252

4

l1l2itg20466

L1, L2

(TA)9

GTAATCATTCATGTATAATTGTAACACTC

CAATTCATTATCTGTATTATTACCCC

180-186

3

Cytochrome B561

l1l2itg53474

L1, L2

(GA)10

CTGAAGTGAGGTTCGGGAAG

TCAATCACACATGCTTGTTCC

230-234

3

Cullin-1

l1l2itg51894

L1

(AT)10

TGACTTTGATTGTTTAGCTTACAGG

TGAATGTCAAATGCAATATTAAGGA

247-263

3

L.hispanicus

l1l2itg24819

L1

(AT)8

CATTCATTCTCTAATCTTTTGTGTCA

TAAAGCTTGTCTCTTGCCCG

219-244

5

L.hispanicus

l1l2itg55310

L1

(TA)9

ACCAAAAGGGTGGGTGAAAT

CCTAACATTTGAACATATTTAAAACAA

277-283

4

l1l2itg14694

L1, L2

(TA)8

AAGTAGGAAGATCGAATATGAACG

GGGAAAATATCGAGGTTTTCATC

268-278

3

L.hispanicus, L.mutabilis

RNA-binding protein

l1l2itg35641

L1

(AT)8

AGTTGCAATTCAACAACGCA

CATGCTCTATGGCAAGTGCT

247-251

3

l1l2itg38340

L1

(TAT)7

AGCTCCACTTTTAGAATTGCG

TCTATTGTTACATGCACATTATCCC

164-173

4

L.hispanicus

l1l2itg26293

L2

(TCCGAA)15

CCTGCAGTGGTAGAACCTGG

GAAGCAAGGTCCACAGAAGG

123-183

6

18S ribosomal RNA gene

l1l2itg42878

L2

(CATTCC)11

CAACTCTTGTTTGCAGACCG

GCTACCCTTTCGGGACTAGC

217-235

4

L.hispanicus, L.mutabilis

l1l2itg13749

L2

(TTCCGC)8

TTTTTACTCGACTCGCTCCC

CCAGTCGATTTAGCAGTCGC

207-261

7

L.hispanicus, L.mutabilis

l1l2itg32760

L1, L2

(CGGAAT)14

TCATAATGAATTAAATTAACCCCC

TCCCTGACTCTGTCTTTGGG

146-284

14

L.hispanicus

l1l2itg00675

L2

(TCT)8(TCG)5

AGAGAGATCCTCTTTGACGCC

GTGGTTAGCGAGAACCATCG

187-199

4

BSD domain-containing protein

l1l2itg45631

L2

(ATC)10

AAACCGAATTGTGGATCAGC

GGGGACTCTGGAAAATCAGG

146-155

3

L.hispanicus, L.mutabilis

Alphavirus core protein family

l1l2itg20349

L2

(AAC)7

ACTAAGGGAAAGGGATTCGG

CCAGGCAAGAACAAAAGAGG

186-189

2

L.hispanicus, L.mutabilis

LPA2 (low psii accumulation2)

l1l2itg41827

L2

(TTG)7

TTGAGTCATATCACCATAGCGG

CAACCACAAATGGAAAACCC

242-245

2

L.hispanicus, L.mutabilis

Lipase class 3 family protein

l1l2itg47916

L2

(TCT)9

GGTGGGTGAAAATGAAATGG

TAACCAAAATGGTTCGTCGG

241-247

2

L.hispanicus, L.mutabilis

l1l2itg42002

L2

(AAC)8

CTTGCAGGGTCTTCTTACAGC

GGGGTTGTTTTTGGTGTCC

243-246

2

L.hispanicus

l1l2itg54849

L2

(ACA)7

TTCTCCAATGATGAAATGCC

TTCACGGCTAAATACCAAGC

177-183

2

L.hispanicus

Microtubule-associated protein

l1l2itg13638

L2

(TGT)9

CCATGGTCATCATTAACCCC

CGAGTCGAGTTCGTTTACCC

188-200

5

L.hispanicus, L.mutabilis

f-box family protein

l1l2itg26640

L2

(AG)7

GGTCTGTTGGAGAAGGCTACC

CCACCAATGGGTAGACATACG

203-209

3

L.hispanicus

Small nuclear ribonucleoprotein

l1l2itg29887

L2

(GCT)10

CCCATCTGAAAGACTTACGGC

TCCCTTTTCATCCAGAGAGG

243-249

2

L.hispanicus

Ser/thr-protein kinase AFC2

l1l2itg50945

L2

(CCA)6(ACA)7

CCAGAACAAGGAGAAGGTTCC

TTCTTCTTCCTCGCAGGC

198-204

3

L.hispanicus

Zinc finger, Transcription factor

l1l2itg44905

L2

(CTT)9

AAATCACAGAGCCAAGGAGG

TCAGCTTATTTTGTTTCCAAGC

356-362

3

L.hispanicus, L.mutabilis

Transcription factor

l1l2itg09113

L2

(AT)8

CATGACCCAATCTCAAACCC

GCATCTGGATCTGCTTAATTGG

341-343

2

L.hispanicus

l1l2itg03938

L2

(CCGATT)9

CATGTGGGAAGACCAGAAGC

ACTACGCGCTGCTAATGTCC

212-290

7

L.hispanicus, L.mutabilis

Polygalacturonase

l1l2itg32421

L2

(AATCGG)8

AGAGAAGTAGGCATGGTGGC

GATCGGCCTATTCACTCAGC

221-293

5

L.hispanicus, L.mutabilis

l1l2itg29217

L1, L2

(AT)7

ACACTCTCAAGGAAAAGGGC

CCATTTAACCGATAATGCTTGG

340-344

2

L.hispanicus

Lactoylglutathione lyase

l1l2itg27515

L2

(TTC)17

CATGCGTCCAATCTATCACC

AGTGGGAAACAAGGAAGTGG

182-221

8

L.hispanicus, L.mutabilis

PPR-containing protein

l1l2itg41211

L2

(GAA)11

TCCTCCTGCTTCAGAACG

AAATCCACGTCATCAATCCG

209-230

6

L.hispanicus, L.mutabilis