Figure 6
From: Small interfering RNA-producing loci in the ancient parasitic eukaryote Trypanosoma brucei
Steady-state mRNA levels at two convergent transcription units in wild-type and DCL2KO cells. (A) and (B) Total RNA was prepared from wild-type and dcl2−/− cells and analyzed by Northern hybridization with a probe specific for the genes indicated below the read distribution. See additional file 3 for sequences of the hybridization probes. The hybridization was quantitated by PhosphorImager analysis, normalized to the loading control (tRNA) and expressed as a ratio between dcl2−/− and wt.