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Figure 1 | BMC Genomics

Figure 1

From: Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum

Figure 1

Genetic map of the sigH-rshA operon, its Northern hybridization analysis in C. glutamicum RES167 and its deletion derivatives. A. Genetic map of the sigH-rshA region showing locations and sizes of deletions in the chromosomes of strains C. glutamicum ΔrshA, DN2 and AS1, predicted sizes of respective sigH-rshA and rshA transcripts (arrows) and locations of probes used for Northern hybridizations. Promoters are indicated with bent arrows and the terminator with a hairpin symbol. B. Northern blot using a sigH probe (left panel) and an rshA probe (right panel) hybridized with total RNA extracted from: RES167 cells (lane 1); DN2 cells (ΔsigH deletion; lane 2); AS1 cells (ΔsigHrshA deletion; lane 3). The estimated lenghts of the detected transcripts (left) and their designations (right) are indicated. The sizes of the fragments in the RNA marker are indicated with arrows.

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BMC Genomics

ISSN: 1471-2164

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