Table 1 Genes with enhanced expression in C. glutamicum Δ rshA compared with C . glutamicum RES167 (reference) sorted by function

Disulphide stress related genes

cg3405◊*

NADPH:quinone reductase

13.27

cg2538

Alkanal monooxygenase (FMN-linked)

12.3

cg2838

Putative dithiol-disulfide isomerase

11.71

cg3236

msrA

Protein-methionine-S-oxide reductase

6.59

cg2194

mtr

Putative NADPH-dependent mycothiol reductase

4.92

cg1709▫^-

mshC

Putative 1-D-myo-inosityl-2-amino-2-deoxy-alpha- D-glucopyranoside—L-cysteine ligase

4.47

cg3299

trxB1

Thioredoxin (TRX)

3.73

cg2078

msrB

Peptide methionine sulfoxide reductase

3.61

cg3422○*

trxB

Thioredoxin reductase

3.53

cg3423○

trxC

Thioredoxin

2.97

cg2661

Putative dithiol-disulfide isomerase

2.68

cg3344

Putative nitroreductase

2.66

cg1127▪

mca

Putative mycothiol S-conjugate amidase

2.46

cg2214

Putative Fe-S-cluster redox enzyme

2.27

cg0497□*

mca

Glutamyl-tRNA reductase

2.07

cg1765

sufR

Transcriptional repressor of suf operon

1.75

cg1553

qor2

quinone oxidoreductase involved in disulfide stress response

1.60

cg1375

Putative thioredoxin

1.58

1

Heat stress-related genes

cg2515

dnaJ2

Chaperone, contains C-terminal Zn-finger domain

1.85

cg2644:

clpP2

Endopeptidase Clp, proteolytic subunit

1.68

cg3079●*

clpB

Putative ATP-dependent protease (heat-shock protein)

1.57

cg2645:*

clpP1

Endopeptidase Clp, proteolytic subunit

1.57

SOS and DNA repair genes

cg1555

uvrD3

DNA/RNA helicase, superfamily I

3.78

cg1560

uvrA

Excinuclease ABC, ATPase subunit A

2.23

cg0184^*

Putative RNA-binding protein

1.95

cg0185^

Putative glyoxalase

1.91

cg0186^

Putative methylated-DNA--protein-cysteine methyltransferase

1.91

cg1795

uvrC

Excinuclease subunit C

1.53

Proteasome components

cg1688°::

pafA2

Putative proteasome component

2.71

cg1689°^::*

pup

prokaryotic ubiquitin-like protein

2.48

cg0998

Trypsin-like serine protease

1.54

Genes with other function

cg3407◊

Putative membrane protein

6.41

cg2106

Conserved hypothetical protein

6.19

cg0617†*

Hypothetical protein

4.20

cg1288

Putative multidrug efflux permease, MFS-type

3.94

cg1432

ilvD

Dihydroxy-acid dehydratase

3.84

cg1398‡

Conserved hypothetical protein

3.78

cg0614†

Hypothetical protein

3.71

cg0616†

fdhD

Putative formate dehydrogenase, FdhD-family

3.71

cg1397‡*

trmU

tRNA (5-methylaminomethyl-2-thiouridylate) -methyltransferase

3.71

cg2423

lipA

Lipoyl synthetase

3.34

cg0378

Putative phage-associated protein

2.93

cg2411

Conserved hypothetical protein, HesB/YadR/YfhF family

2.81

cg1799·*

ribC

Riboflavin synthase, alpha chain

2.73

cg2247

Hypothetical protein

2.41

cg1282

Conserved hypothetical protein

2.35

cg2127

Hypothetical protein

2.35

cg3424○

cwlM

N-Acetylmuramoyl-L-alanine amidase

2.33

cg1798·

ribA

Putative GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphatesynthase

2.22

cg2835

Putative acetyltransferase

2.17

cg1281

ABC-type putative multidrug transporter, ATPase and permease subunit

2.16

cg1687°^::

Putative transcriptional regulatory protein

2.13

cg1797·

ribH

Riboflavin synthase, beta chain

2.10

cg1779

opcA

Glucose-6-phosphate 1-dehydrogenase subunit

2.06

cg2762

murI

Glutamate racemase

2.03

cg3078●

Hypothetical protein

1.97

cg1411°

rbsA

ABC-type ribose transporter, ATPase subunit (TC 3.A.1.2.1)

1.92

cg2636

catA

Catechol 1,2-dioxygenase

1.88

cg1780

pgl

6-Phosphogluconolactonase

1.87

cg1413°

rbsB

ABC-type ribose transporter, substrate-binding lipoprotein (TC 3.A.1.2.1)

1.85

cg0498□

hemC

Porphobilinogen deaminase

1.84

cg2665

Hypothetical protein

1.82

cg2181†*

ABC-type putative dipeptide/oligopeptide transporter, substrate-binding lipoprotein

1.80

cg1128▪

Hypothetical protein, similar to ribosomal protein S2

1.79

cg1139

Allophanate hydrolase subunit 2

1.75

cg1708^-

Conserved hypothetical protein

1.75

cg2560

aceA

Isocitrate lyase

1.72

cg2183†

ABC-type putative dipeptide/oligopeptide transporter, permease subunit

1.71

cg2434

Putative monooxygenase, luciferase

1.68

cg0380

Hypothetical protein

1.67

cg0043

ABC-type putative manganese/zinc transporter, ATPase subunit

1.65

cg0228

Two-component system, sensory histidine kinase, putative pseudogene

1.65

cg1412°

rbsC

ABC-type ribose transporter, permease subunit (TC 3.A.1.2.1)

1.65

cg1778

zwf

Glucose-6-phosphate 1-dehydrogenase

1.65

cg1686°

Putative transcriptional regulatory protein

1.61

cg1482

Putative Zn-dependent hydrolase

1.61

cg2514

Conserved hypothetical protein

1.59

cg2206

ispG

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

1.58

cg2546

Putative secondary C4-dicarboxylate transporter, tripartite ATP-independent transporter (TRAP-T) family

1.58

cg0699

guaB2

IMP dehydrogenase

1.56

cg2184

ABC-type putative dipeptide/oligopeptide transporter, ATPase subunit

1.56

cg3077●

Putative membrane protein

1.56

cg1410°*

rbsR

Transcriptional repressor of ribose importer RbsACBD, LacI-family

1.54

cg1464

Putative transcriptional regulator, HTH_3-family

1.52