Figure 3

Differentially expressed miRNAs between CB- and PB-EPCs discovered by smRNA-Seq. ( A) A table summarizes reads number ( upper) and a flowchart describes the data analysis pipeline for quantification of known miRNAs from smRNA-Seq data ( lower). ( B) Differential expressed miRNAs between cord blood EPCs and adult peripheral blood EPCs. ( C-D) qPCR validation of smRNA-Seq data. Mean miRNA expression levels were compared to the average CT values of U6 snRNA + U48 snRNA + 5S rRNA controls. miRNAs more abundant in CB-EPC ( C) or PB-EPC ( D) were verified. *:P < 0.05 ( E) A major functional genetic network composed of multiple PB-EPC microRNAs (in green) and CB-EPC genes (in red). This network is displayed graphically as nodes (gene products) and edges (biological relationships between nodes) mapped by the Ingenuity Pathway Analysis (IPA) tool. The intensity of the node color indicates the degree of differential expression. Hub miRNAs in this genetic network are shown.