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Table 5 Proteins differentially regulated by diet

From: Effects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar)

Spot No Protein ID Accession No. (NCBInr) Theoretical Mw (KDa)/pI Protein Scorea No. Matched peptides (MS/MS)b Best score peptide VO/FO Lean VO/FO Fat p-value
1136 Epoxide hydrolase 2 (EPHX2) gb|ACI33129.1| 54.2/5.52 84 1 GGLFVGLPDEIPR 1.23 1.40 0.0190
1148 Hemopexin-like protein* (HPX) emb|CAA92147.1| 50.4/5.61 169 1 VHLDAITSDDAGNIYAFR 1.32 1.56 0.0002
1151 Hemopexin-like protein* (HPX) ref|NP_001104617.1| 51.0/6.18 161 1 VHLDAITSDDAGNIYAFR 1.38 1.54 0.0001
2683 Peroxiredoxin 1 (PRDX1) gb|ACI67145.1| 22.0/6.42 757 8 SISTDYGVLKEDEGIAYR 1.14 1.09 0.0310
3168 2-peptidylprolyl isomerase A (PPIA) ref|NP_001135161.1| 17.5/7.61 101 1 VYFDITIGDTPAGR −1.46 −1.18 0.0170
3243 Myosin light chain smooth muscle isoform (MYL) ref|NP_998803.1| 16.9/4.47 100 2 EAFLLFDR −1.23 −1.11 0.0490
3331 similar to H2A histone family, member J (H2A) ref|XP_001521566.1| 13.3/10.84 74 1 AGLQFPVGR −1.58 −1.18 0.0250
3334 Histone H2A** (H2A) emb|CAA25528.1| 13.7/10.88 37 1 AGLQFPVGR −1.48 −1.18 0.0210
3445 Galectin 2 (LGALS2) gb|ACN10131.1| 14.8/5.93 314 2 SGASSFSINIGHDSDNYALHFNPR 1.17 1.15 0.0170
  1. a The protein score probability limit (where P < 0.05) is 73.
  2. b Peptides with confidence interval above 95% were considered.
  3. * Equivalent to warm-temperature-acclimation-related-65 kDa-protein.
  4. **Same protein identification obtained by MS/PMF (peptide mass fingerprinting): Score = 76; 7 peptides matched.
  5. Only reliable identifications of Actinopterygii, obtained by mass spectrometry (MALDI-TOF-MS/MS) analysis and searches in MASCOT, NCBI and ExPASy (Mw/pI) databases are shown. Expression ratios between fish fed VO and FO diets are given for each family group, as well as p-value for diet (two-way ANOVA, DeCyder V7.0).