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Table 6 Proteins differentially regulated by genotype

From: Effects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar)

Spot No Protein ID Accession No. (NCBInr) Theoretical Mw (KDa)/pI Protein Scorea No. Matched peptides (MS/MS)b Best score peptide Lean/Fat FO Lean/Fat VO p-value
1148 Hemopexin-like protein* (HPX) emb|CAA92147.1| 50.4/5.61 169 1 VHLDAITSDDAGNIYAFR −1.09 −1.29 0.0270
1389 Calreticulin precursor (CALR) gb|ACI32936.1| 47.6/4.33 81 2 FEPFSNEGK −1.28 −1.33 0.0026
1441 Alpha-enolase (ENO1) ref|NP_001133366.1| 47.0/5.91 405 3 AAVPSGASTGIYEALELR 1.28 1.2 0.0066
1714 Heat shock protein 70 (HSP70) ref|NP_990334.1| 70.8/5.47 111 1 IINEPTAAAIAYGLDKK 1.16 1.77 0.0260
2134 Heterogeneous nuclear ribonucleoprotein A0 (HNRNPA0) gb|ACI67551.1| 28.8/9.10 235 2 LFVGGLNVDTDDDGLRK −1.19 −1.09 0.0460
2154 Calponin 1 (CNN1) ref|NP_001139857.1| 33.2/8.56 565 4 KINTSPQNWHQLENIGNFVR −1.34 −1.51 0.0350
2157 Calponin 1*** (CNN1) ref|NP_001139857.1| 33.2/8.56 43 1 YDPQKEEELR −1.34 −1.47 0.0240
2213 Caspase 3 (CASP3) ref|NP_001133393.1| 31.0/5.97 254 2 IPVEADFLYAYSTAPGYYSWR −1.15 −1.24 0.0300
2215 Caspase 3 (CASP3) ref|NP_001133393.1| 31.0/5.97 279 2 VANDQTVQQIQQLLSK −1.14 −1.31 0.0130
2282 Voltage-dependent anion channel 2–2 (VDAC2) gb|ACH71030.1| 30.1/8.85 214 1 VNNNSLVGVGYTQTLRPGVK −1.24 −1.19 0.0160
2458 Annexin A4 (ANXA4) gb|ACI69495.1| 28.4/5.22 218 3 NHLLQVFK −1.14 −1.11 0.0300
2487 Dihydropteridine reductase (DHPR) gb|ACI67281.1| 15.7/8.46 81 1 QSVWTSTISSHLATR −1.14 −1.11 0.0300
2499 Pancreatic alpha-amylase precursor (AMY2) ref|NP_001036176.1| 57.4/6.89 86 1 ALVFVDNHDNQR −1.37 −1.27 0.0021
2522 Triosephosphate isomerase 1b (TPI1) ref|NP_001133174.1| 26.6/7.63 336 2 LDPNTEVVCGAPSIYLEFAR 1.26 1.16 0.0009
2527 Histone cluster 1 (H2A) ref|NP_001086775.1| 14.0/10.88 94 1 AGLQFPVGR 1.12 1.11 0.0074
2677 Transgelin (TAGLN) gb|ACM09025.1| 21.7/7.69 219 1 DGCVLSELINSLHK −1.61 −1.35 0.0350
2717 Proteasome beta 1 subunit (PSMB1) ref|NP_001003889.1| 26.1/6.32 87 1 GAVYSFDPVGSYQR −1.29 −1.12 0.0140
2903 Peroxiredoxin-1(PRDX1) gb|ACI67145.1| 22.0/6.42 115 1 QITINDLPVGR −1.62 −1.99 0.0069
3345 Retinol-binding protein II, cellular (RBP2) ref|NP_001139954.1| 15.6/5.44 70 2 AIDIDFATR −1.32 −1.1 0.0330
3457 Alpha globin (HBA) emb|CAA65949.1| 15.1/9.19 170 2 TYFSHWADLSPGSAPVK 1.03 2.09 0.0320
  1. aThe protein score probability limit (where P < 0.05) is 73.
  2. b Peptides with confidence interval above 95% were considered.
  3. * Equivalent to warm-temperature-acclimation-related-65 kDa-protein.
  4. *** Same protein identification obtained by MS/PMF (peptide mass fingerprinting): Score = 193; 27 peptides matched.
  5. Only reliable identifications of Actinopterygii, obtained by mass spectrometry (MALDI-TOF-MS/MS) analysis and searches in MASCOT, NCBI and ExPASy (Mw/pI) databases are shown. Expression ratios between Lean and Fat family groups are given for each dietary treatment, as well as p-value for genotype (two-way ANOVA, DeCyder V7.0).