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Table 1 Primers used for semi-quantitative RT-PCR

From: Serpins in rice: protein sequence analysis, phylogeny and gene expression during development

Primer Sequence Amplicon size (bp)
OsSRP-QKG fwd 5-TGCCCCGAGCCGCATTCTAC-3 359
 rvs 5-TTGCATCATAACCACGGCGG-3  
OsSRP-LGC fwd 5-GACACCGGCCGCCTCTTCTC-3 383
 rvs 5-GCGCAGCCAAGGGTCATGAC-3  
OsSRP-LRS fwd 5-AAGCTTCCATACCAGCAAGG-3 395
 rvs 5-GGAGCTGACCTAAGTGTGATC-3  
OsSRP-FRS fwd 5-GCTTATGGCTTGACCACAAG-3 451
 rvs 5-CACTGCCTTCCATCACGTAG-3  
OsSRP-PTY fwd 5-ACGACGGCCAGGTCCACTTC-3 398
 rvs 5-GCTATAGGTCGGGCTGCAAC-3  
OsSRP-PLP fwd 5-AGCAAGGAAAGAATGAAAGG-3 362
 rvs 5-CAAGCCCATTGATACTGAAG-3  
OsSRP-FAS fwd 5-GCTTGTGTGAGCGCGAGGAC-3 399
 rvs 5-GAAGGTAAGACAAACCGCGG-3  
OsSRP-FLC fwd 5-GATGGTGGTGACATCACTCC-3 423
 rvs 5-CAAGAACTTCATCGTGCAGG-3  
Actin fwd 5-TCCATCTTGGCATCTCTCAG-3 418
 rvs 5-GTACCCGCATCAGGCATCTG-3  
  1. BLASTN searches against the rice genome using the primers as query sequences were performed to check for the possibility of non-specific hybridisation. fwd = forward; rvs = reverse.