Time-course microarrays reveal early activation of the immune transcriptome and adipokine dysregulation leads to fibrosis in visceral adipose depots during diet-induced obesity

Table 2 Functional annotation gene clusters (A) up-regulated and (B) down-regulated in visceral adipose tissue depots by high-fat feeding in C57BL/6 J mice over 24 weeks

	Depot	E.S.	Functional terms	No. of genes	p-value
(A)	Epididymal WAT	5.34	Inflammatory response	41	1.10E-06
			Response to wounding	53	6.40E-06
			Defense response	62	8.20E-06
		2.59	Regulation of phagocytosis	10	4.20E-05
		2.23	Cell activation	38	5.60E-05
		2.09	Positive regulation of immune system process	37	2.30E-06
			Positive regulation of immune response	25	8.40E-05
	Mesenteric WAT	4.84	Positive regulation of immune system process	32	3.80E-08
			Positive regulation of immune response	25	5.40E-08
			Activation of immune response	17	4.40E-06
		3.48	Defense response	47	3.40E-06
		2.72	Cell activation	32	2.10E-06
			Leukocyte activation	28	1.50E-05
			Lymphocyte activation	25	3.10E-05
(B)	Epididymal WAT	5.77	Fatty acid metabolic process	20	1.60E-08
		3.55	Hexose metabolic process	20	2.80E-09
			Monosaccharide metabolic process	21	3.40E-09
			Glucose metabolic process	17	3.40E-08
	Mesenteric WAT	12.62	Glucose metabolic process	36	2.80E-18
			Monosaccharide metabolic process	40	8.20E-17
			Hexose metabolic process	37	4.80E-16
			Glycolysis	17	7.60E-12
			Gluconeogenesis	7	9.10E-05
		4.41	Steroid metabolic process	26	3.80E-08
			Sterol metabolic process	16	9.10E-07
			Cholesterol metabolic process	15	1.50E-06
			Lipid biosynthetic process	32	1.90E-06
			Lipid catabolic process	20	5.60E-06
			Acetyl-CoA metabolic process	9	3.70E-05

Functional annotation terms enriched amongst the up/down regulated genes in epididymal and mesenteric WAT of HFD fed C57BL/6 J mice over 24 weeks. Functional annotation terms were clustered according to biological processes. WAT: white adipose tissue; E.S.: Enrichment Score.

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