Figure 5
The effects of PML knockdown and/or TNF α on leukocyte:HUVEC adhesion. (a) Clustering analysis of the log change fold values of the coregulatory network of genes significantly affected by PML knockdown and/or TNFα signaling. Using dynamicTreeCut R package as described in Methods, 4 sub-clusters of significantly altered genes were identified and annotated by color side bars (blue, red, green and purple respectively). Control siRNA, “siC”; two PML siRNAs, “siP1” and “siP2”; vehicle treated samples, “U”; TNFα treated samples, “T”; minus sign of “-”, comparison between two samples; sign of “∗”, interaction effects. (b) Quantification of the adherence of human leukocyte U937 cells on HUVECs transfected with control siRNA (siCtrl) or two independent PML siRNAs (siPML-1 and siPML-2) without or with TNFα treatment. Two-tail unpaired t-tests: ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.