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Table 5 Correlation between differences in carbon source utilisation and genotype of B. pilosicoli 95/1000, B2904 and WesB

From: Comparative genomics of Brachyspira pilosicoli strains: genome rearrangements, reductions and correlation of genetic compliment with phenotypic diversity

Unique carbon source compound tested

95/1000

B2904

WesB

Possible explanation

D-Mannose

-

-

+

WesB is the only strain with the mannose/sorbose-specific PTS system IIABCD components (wesB_1269, wesB_1270, wesB_1271 and wesB_1272) for uptake and phosphorylation of D-mannose.

D-Glucuronic acid

-

+

+

95/1000 lacks the pfkB carbohydrate kinase, 2-dehydro-3-deoxygluconate kinase, which links D-glucuronic acid metabolism to glycolysis. This enzyme is found in both B2904 (B2904_orf899 and B2904_orf900) and WesB (wesB_1781).

D-Mannitol

-

+

-

B2904 is the only strain with the D-mannitol PTS system IIABC components (B2904_orf2447) and also a mannitol-1-phosphate 5-dehydrogenase (B2904_orf2446) for D-mannitol, uptake, phosphorylation and catabolism.

Glucuronamide

-

+

+

95/1000 lacks the pfkB carbohydrate kinase, 2-dehydro-3-deoxygluconate kinase, which links glucuronate and related compound metabolism to glycolysis. This enzyme is found in both B2904 (B2904_orf899 and B2904_orf899 and B2904_orf900) and WesB (wesB_1781).

β-D-Allose

-

-

+

WesB is the only strains with D-allose ABC transporter components (wesB_1171, wesB_1172 and wesB_1175) and D-allose kinase (wesB_0259 and wesB_1174) for uptake and metabolism of D-allose.

β-Methyl-D-glucuronic acid

-

+

+

95/1000 lacks the pfkB carbohydrate kinase, 2-dehydro-3-deoxygluconate kinase, which links glucuronate and related compound metabolism to glycolysis. This enzyme is found in both B2904 (B2904_orf899 and B2904_orf900) and WesB (wesB_1781).

L-Sorbose

-

-

+

WesB is the only strain with the mannose/sorbose-specific PTS system IIABCD (wesB_1269, wesB_1270, wesB_1271, wesB_1272) components for uptake and phosphorylation of L-sorbose.

  1. Possible explanations for the differences in phenotype relate to differences in genomic features.