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Table 4 Gene expression after real-time PCR.

From: Identification of differentially expressed genes in SHSY5Y cells exposed to okadaic acid by suppression subtractive hybridization

 

Control

OA (100 nM)

 

3 h treatment

 

NEFM

0.7 ± 0.1

0.1 ± 0.0*

TUBB

1.6 ± 0.4

1.0 ± 0.4

SEPT7

1.7 ± 0.4

1.0 ± 0.1*

SYT4

1.4 ± 0.0

0.3 ± 0.0**

NPY

0.6 ± 0.2

0.3 ± 0.0*

 

24 h treatment

 

NEFM

1.6 ± 0.0

5.6 ± 0.6*

TUBB

1.5 ± 0.1

0.4 ± 0.0**

SEPT7

1.2 ± 0.1

0.9 ± 0.2

SYT4

2.8 ± 0.1

0.7 ± 0.2*

NPY

2.3 ± 0.3

1.3 ± 0.1*

 

48 h treatment

 

NEFM

1.3 ± 0.1

0.8 ± 0.3

TUBB

1.3 ± 0.1

1.5 ± 0.7

SEPT7

0.6 ± 0.0

0.7 ± 0.0

SYT4

0.9 ± 0.1

0.9 ± 0.0

NPY

1.1 ± 0.1

1.2 ± 0.1

  1. *P < 0.05, **P < 0.01, significant difference with regard to the expression of reference gen.