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Table 5 Primers and PCR conditions for generation of the digoxigenin-labeled probes

From: Tracing genomic variations in two highly virulent Yersinia enterocolitica strains with unequal ability to compete for host colonization

Primer

Sequence

Probe

PCR conditions

Hem_8081_FW

5'-CAATATGACTACCGACCCGGTTAC-3'

Hem_8081

- Denaturation at 95°C for 2 min

Hem_8081_RV

5'-GGATACATCTGCTGGGCGATATAC-3'

162 bp

- 30 cycles:

denaturation at 95°C for 30 sec

Col_WA_FW

5'-CGATCGTAGTAGTAAGGCAACTCC-3'

Col_WA

annealing at 60°C for 30 sec

Col_WA_RV

5'-GACGGTATCATGCCCATAACTG-3'

234 bp

elongation at 72°C for 40 sec

- Final elongation at 72°C for 7 min