Whole Genome Profiling provides a robust framework for physical mapping and sequencing in the highly complex and repetitive wheat genome

Table 1 Characteristics of the sequence tags produced by WGP

	Expected data	Raw data	Tag filtering	BAC filtering
Total number of tags	5,681,912	327,282	228,263	194,716
Total number of unique tags	97,293^a	111,678	47,900	47,220
Average tag length (bp)	30.0	30.0	29.9	29.9
Number of BAC with tag	16,128	14,199	13,888	11,238
Average number of tag per BAC	58.4^b	23.0	16.4	17.3
Average number of BAC sharing the same tag	9.6	2.9	4.8	4.1

^a based on a average Eco RI sites density of 4,728 bp and a coverage of the BACs subset of 230 Mb.
^b based on a average Eco RI sites density of 4,728 bp and an average BAC size of 138 Kb.

ISSN: 1471-2164