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Table 1 Primer sequences for real-time qPCR validation

From: Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (Sparus aurata), but modulate the transcriptomic response to infection with Enteromyxum leei

Gene name Symbol Primer sequence
Arginase-1 ARG1 F CGT CCA GTC CAC AGT CAG CAC
R TCG GGC AGG CGG TAG TCC
B(0,+)-type amino acid transporter 1 BAT1 F GCC GTG TGT GCT TTG TTG CTG
R GGT GAA GAT AAG GGC TGG AGA TGG
Complement C1q tumor necrosis factor-related protein 3 C1QTNF3 F ATG CTG TGC TGA GAG AGA TGA G
R AGT CTT CTG CTT CTC CTG CTC
Cytochrome P450 1A1 CYP1A1 F GCA TCA ACG ACC GCT TCA ACG C
R CCT ACA ACC TTC TCA TCC GAC ATC TGG
Fatty acid-binding protein, intestinal FABP2 F CGA GCA CAT TCC GCA CCA AAG
R CCC ACG CAC CCG AGA CTT C
Glucose regulated protein 75 GRP75 F TCC GGT GTG GAT CTG ACC AAA GAC
R TGT TTA GGC CCA GAA GCA TCC ATG
Growth hormone receptor type I GHR-I F ACC TGT CAG CCA CCA CAT GA
R TCG TGC AGA TCT GGG TCG TA
Growth hormone receptor type II GHR-II F GAG TGA ACC CGG CCT GAC AG
R GCG GTG GTA TCT GAT TCA TGG T
Immunoglobulin lambda-like polypeptide 1 IGLL1 F TGA GTG GTG TGA CGG TGG TG
R ATG GTG GCT GTC TCT CCT TTG G
Immunoglobulin M heavy chain IGHM F ACC TCA GCG TCC TTC AGT GTT TAT GAT GCC
R CAG CGT CGT CGT CAA CAA GCC AAG C
Insulin-like growth factor-II IGF-II F TGG GAT CGT AGA GGA GTG TTG T
R CTG TAG AGA GGT GGC CGA CA
Insulin-like growth factor-binding protein 4 IGFBP4 F GGC ATC AAA CAC CCG CAC AC
R ATC CAC GCA CCA GCA CTT CC
Interleukin-6 IL6 F TCT TGA AGG TGG TGC TGG AAG TG
R AAG GAC AAT CTG CTG GAA GTG AGG
Interleukin-6 receptor subunit beta IL6Rβ F AGC ACT GAG TCT CCG TAT GAA GC
R ACA ACT GAA ACC GCA TCT AAA GGC
Liver X receptor alpha LXRα F GCA CTT CGC CTC CAG GAC AAG
R CAG TCT TCA CAC AGC CAC ATC AGG
Metallothionein MT F CTC TAA GAC TGG AAC CTG
R GGG CAG CAT GAG CAG CAG
Ornithine aminotransferase OAT F TGC GGT CTG AGC TGA ACA A
R CTT CCA GGC GTC GTA GTC T
Ornithine decarboxylase ODC F GCC TCG TGT CAC TCC CTT CTA TG
R GCT GAA TCT CCG TCT TGC TTG C
Peroxiredoxin 1 PRDX1 F CTC CAA GCA ATA ATA AGC CCA AAG
R TCA CTC TAC AGA CAA CAG AAC AC
Peroxiredoxin 6 PRDX6 F AGA GAC AAG GAC GGA ATG C
R TGT GGC GAC CTT CTT CTG
Uncoupling protein 1 UCP1 F GCA CAC TAC CCA ACA TCA CAA G
R CGC CGA ACG CAG AAA CAA AG
ß-Actin   F TCC TGC GGA ATC CAT GAG A
   R GAC GTC GCA CTT CAT GAT GCT