Enhanced genome annotation, single nucleotide variation analyses, and differentially expressed genes before and after exercise in horse. (A) Red and green circles indicate expressed genes in the blood and muscle tissues, respectively, and the blue circle shows the current Ensembl annotation (Release 62). The grey rectangle indicates the coverage of the current horse genome. (B) Green circle: SNPs provided by the Broad Institute, red circle: SNPs provided by Ensembl (release 62), blue circle: SNPs identified from this study. (C) SNV profiles of six horses for the titin (TTN) gene. The top of the blue arrow is the 5' end and the bottom is 3' end of TTN gene. The X-axis shows the names of the horses. Dark green horizontal bars are non-synonymous SNVs. Light green horizontal bars are synonymous SNVs. (D) Blue bars: >2-fold upregulated genes, red bars: >2-fold downregulated genes, white bars: not differentially expressed. The four pie charts display the composition of the DEGs supported by four horses (white), five horses (light grey), and six horses (grey).