Figure 6

Comparison of expression changes in RNA-Seq and microarray-based methods. The log₂ fold-change in expression values measured by the RNA-Seq based method presented here are plotted against those measured by microarray analysis (microarray data from [16]). The red spots are representative of the full set of 2,290 sequences from the microarray for which a best hit was selected from the de novo assembly. The blue spots indicate sequences that were statistically significant in both comparisons to a threshold of p < 0.01. Three sequences are not shown that were down-regulated in the microarray analysis but seemed to be turned off completely in the RNA-Seq analysis as a result of exposure. Another four sequences are not shown which would have expanded the scale greatly but for which both techniques agreed in the direction of regulation if not the magnitude. A linear model is fit to the blue points, and the Pearson’s correlation is given as well.