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Figure 4 | BMC Genomics

Figure 4

From: Genome sequencing and genetic breeding of a bioethanol Saccharomyces cerevisiae strain YJS329

Figure 4

The effects of genomic variations on the transcriptional differences between BYZ1 (orange) and YJS329 (green). (A) Comparison of expression levels of HSF1 in BYZ1 and YJS329 within a sliding window of 50 bp. The N-terminal activation domain (NAD), DNA-binding domain (DBD), trimerization domain (TD), and C-terminal activation domain (CAD) of the Hsf1p [33] are highlighted by colored boxes. The orange letters represent the corresponding amino acids in BYZ1; the olive letters represent those in YJS329. (B) Comparison of the promoter and the expression levels of the SFA1 gene in BYZ1 and YJS329. The green box in the SFA1 promoter represents the Msn2/4p binding motif in YJS329. (C) The insertion of a Ty2 element into the CTR3 promoter greatly decreased the expression of the CTR3 gene in BYZ1 (sliding window of 50 bp). (D) The down-regulation of ALD6 in YJS329 might be caused by the loss of the Adr1p binding motif in the promoter (sliding window of 50 bp). (E) The relative expression level of the amplified region located on chromosome 4 of BYZ1, represented by the log2 ratio (BYZ1/YJS329), within a sliding window of 100 bp. The red dotted line indicates the mean value of the relative expression level. The up-regulated genes in the amplified region are indicated by violet boxes (P < 0.001); the genes that were not differentially expressed in this region are indicated by yellow boxes (P > 0.001).

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