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Table 1 Effects of biological replication on power to detect DE using DESeq

From: Efficient experimental design and analysis strategies for the detection of differential expression using RNA-Sequencing

%

n = 2

n = 3

n = 4

n = 6

n = 8

n = 12

call rate %

0.44

1.15

1.76

3.03

4.08

5.12

FPR %

0.04

0.06

0.06

0.06

0.05

0.04

TPR %

3.26

8.95

13.95

24.30

32.72

41.57

  1. Effects of biological replication on power to detect DE using DESeq. FPR and TPR are defined in Eqs. 5 & 6 respectively at 1%. “call rate” is the total number of reported positives / the total number of transcripts. These values are also represented in Figure3 at 100% sequencing depth.
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BMC Genomics

ISSN: 1471-2164

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