Table 5 Primers used for recombinant plasmids construction
From: Mining and characterization of ubiquitin E3 ligases expressed in the mouse testis
Name | Vector | Sequence (5'-3') |
|---|---|---|
Nsmce1 | pGEX-4 T1 | Sense: CG GGATCC ATGGCTTCCGGCGTGATC |
Antisense: CG GAATTC CTAGTGCTGCCGGGTCCGTAA | ||
| Â | pEGFP-N1 | Sense: CG GAATTC ATATGGCTTCCGGCGTGATC |
Antisense: CG GGATCC TTGTGCTGCCGGGTCCGTAA | ||
| Â | pFLAG-CMV4 | Sense: CG GAATTC TATGGCTTCCGGCGTGATC |
Antisense: CG GGATCC CTAGTGCTGCCGGGTCCGTAA | ||
Rnf126 | pGEX-4 T1 | Sense: CG GGATCC ATGGCCGAGGCGTCGCCG |
Antisense: CG GAATTC TCAGGAGTTGCTTGTGGCG | ||
| Â | pEGFP-N1 | Sense: CG GAATTC ATATGGCCGAGGCGTCGCCG |
Antisense: CG GGATCC TTGGAGTTGCTTGTGGCG | ||
| Â | pFLAG-CMV4 | Sense: CG GAATTC TATGGCCGAGGCGTCGCCG |
Antisense: CG GGATCC TCAGGAGTTGCTTGTGGCG | ||
Rnf151 | pGEX-4 T1 | Sense: CCG GGATCC TTCCTGTGTTCTGTCTGCCA |
Antisense: GC CTCGAG CCTTCATAAGACACTTTGGC | ||
| Â | pFLAG-CMV4 | Sense: CG GAATTC GATC ATGAGTGGTGG |
Antisense: CG GGATCC TCATAAGACACTTTGGC | ||
Znrf4 | pEGFP-N1 | Sense: CCG GAATTC CCATGGCGCGGTTTGCGTG |
Antisense: CCG GGATCC CTGGAG AGCT CGGA AGT CGC TTC | ||
| Â | pFLAG-CMV4 | Sense: CCG GAATTC CATGGCGCGGTTTGCGTG |
| Â | Â | Antisense:CCG GGATCC TCAGGAGAGCTCGGAAGTCGC TTC |