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Table 4 Primer details

From: Next generation sequencing and comparative analyses of Xenopus mitogenomes

Name

Gene

Nucleotide position

Sequence (5’-3’)

Amplicon length (bp)

Annealing temp. (°C)

LongF1

trnF _atp6/atp6

28-49

ACTGAAGATGCTGAGATGAGCC

7961

55

LongR2

8012-8033

ATGGTCAGTTTCAAGGGTTAGG

LongF2

atp6/atp6 _trnF

8012-8033

CCTAACCCTTGAAACTGACCAT

9649

55

LongR1

28-49

GGCTCATCTCAGCATCTTCAGT

16SA-Lmod

rrnL

1943-1962

CGCCTGTTTACCAAAAACAT

580

53

16SA-H

 

2542-2562

CCGGTCTGAACTCAGATCACG

 

COX1F

cox1

6613-6631

GAAACATGAGCAAAAATCC

190

53

COX1R

 

6821-6802

AATGCTTCTCAGATAATGAA

 

ND6F

nad6

13996-14015

AACATCCCACCTAAATAAAT

106

53

ND6R

14137-14122

TAGCTGTTGCTTCAAATCC

AMP1F

trnF _rrnS

5-23

ACGTAGCTTAAGTAAAGCACAGC

294

58

AMP1R

322-347

ATCAACTTGAGTTTCTCGTATAACC

AMP2F

cox2_trnK_atp6/cox3

7776-7800

TCTTCATCAATACTAGAAGCCTCA

912

61

AMP2R

8712-8731

TGTGCTTGGTGTGCCATTA

  1. The names, gene and nucleotide positions, sequences, expected amplicon lengths and annealing temperatures of PCR primers used to generate the long-amplicons and to verify the specific identity of the long-amplicon and sequences of the primer regions.