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Figure 1 | BMC Genomics

Figure 1

From: Accurate variant detection across non-amplified and whole genome amplified DNA using targeted next generation sequencing

Figure 1

Experimental Workflow and Study Design. This Figure shows the tested samples (individual, pooled, non-amplified gDNA, and matched WGA) using the established T-NGS workflow (for more details see Additional file 3: Table S1). Each enriched individual sample was barcoded and sequenced under the following conditions: 1) one sample per octet, 3) samples were pooled after SOLiD library construction, pre-emulsion PCR (emPCR), and 3) samples were pooled post-emPCR. To assess the reproducibility of the established T-NGS method, equimolar amounts of the enriched six HapMap samples were also pooled before SOLiD library construction and tested in duplicate (Libraries ID 768_1L and 768_2L are technical replicates).

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BMC Genomics

ISSN: 1471-2164

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