Skip to main content

Advertisement

Table 1 HFA and SRSF genome coverage and hit selection

From: Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway

  HFA screen SRSF screen
  FlyBase r2.0 FlyBase r5.24
  Nr Genes % of genome Nr Genes % of genome
Initial Library coverage 13,226 88.8% 14,587 97.9%
Genes excluded due to screening errors (Figure 1B, step 2) 820 6.2% 0 0.0%
Remaining genome coverage 12,406 83.3% 14,587 97.9%
Genes with significant Average FL/RL (Figure 1B, step 4) 1,161 7.8% 300 2.0%
Final hits after applying all hit selection rules (Figure 1B, step 6) 134 0.9% 42 0.3%
  1. The number of genes and % of the r 5.24 genome (14,898 genes) this represents are shown for each stage of the analysis pipeline. Genome annotation release used in the design of each library is indicated.