Alignment of the amino acid sequence of European eel CYP3A and other vertebrate CYP3A members with high amino acid sequence similarity using the CLUSTAL_W method. Amino acids shared by all species are indicated by a stark mark and amino acids shared by all fish species by a dot mark. Putative recognition sites SRS are indicated by light shade. A gene tree was constructed using the Neighbour-Joining tree method in MEGA5. Genbank accession numbers for the sequences used are as follows: zebrafish CYP3A65, AY452279; killifish CYP3A330, AF105068; medaka CYP3A40, AF251271; rainbow trout CYP3A27, U96077; human CYP3A4, NP059488.