Figure 4

Microscopic analysis of egfp expression under the control of ppg1 regulatory regions. Plasmids pDS23 and pSNPTppg1 were transformed into the S. macrospora wild-type. Strain S106352 expresses egfp under the control of the Aspergillus nidulans gpd promoter and trpC terminator from pDS23 (black). Strains T71.1S29 and T75.10xfusS44 express egfp under the control of the S. macrospora ppg1 promoter and terminator regions from pSNPTppg1. Note that EGFP fluorescence under the control of ppg1 regulatory regions in protoperithecia was observed through a 6% neutral density filter because of very strong fluorescence, which is consistent with high ppg1 expression in these structures. GFP images are z projections of stacks spanning whole protoperithecia with a plane distance of 0.5 μm. DIC images show the middle plane of the corresponding stack. Scale bar, 10 μm.