Table 3 Distribution of the SSR frequency in Brassica EST sequences
Motifs | Number | Percentage (%) | Total length (bp) | Average length (bp) |
|---|---|---|---|---|
Mononucletide | 339538 | 76.277 | 7614099 | 22.42 |
 A | 310831 | 69.828 | 7256120 | 23.34 |
 C | 28707 | 6.449 | 357979 | 12.47 |
Dinucleotide | 55734 | 12.521 | 935952 | 16.79 |
 AG | 44403 | 9.975 | 756440 | 17.04 |
 AT | 6090 | 1.368 | 99192 | 16.29 |
 AC | 5186 | 1.165 | 79618 | 15.35 |
 CG | 55 | 0.012 | 702 | 12.76 |
Trinucleotide | 48202 | 10.829 | 818475 | 16.98 |
 AAG | 17256 | 3.877 | 298896 | 17.32 |
 AGG | 7389 | 1.660 | 123783 | 16.75 |
 ACT | 5199 | 1.168 | 88563 | 17.03 |
 ACC | 3878 | 0.871 | 62421 | 16.10 |
 AAC | 3644 | 0.819 | 61962 | 17.00 |
 AGT | 3433 | 0.771 | 58869 | 17.15 |
 AGC | 2305 | 0.518 | 38754 | 16.81 |
 ACG | 2063 | 0.463 | 33597 | 16.29 |
 CCG | 1558 | 0.350 | 25122 | 16.12 |
 AAT | 1477 | 0.332 | 26508 | 17.95 |
Tetranucleotide | 1026 | 0.230 | 23372 | 22.78 |
 AAAG | 249 | 0.056 | 5784 | 23.23 |
 AAAC | 164 | 0.037 | 3484 | 21.24 |
 AAGG | 84 | 0.019 | 1912 | 22.76 |
 AACG | 82 | 0.018 | 1844 | 22.49 |
 Others | 447 | 0.100 | 10348 | 23.15 |
Pentanucleotide | 220 | 0.049 | 5980 | 27.18 |
 AAACC | 40 | 0.009 | 1195 | 29.88 |
 AAAAG | 36 | 0.008 | 935 | 25.97 |
 AGAGG | 21 | 0.005 | 570 | 27.14 |
 AAAAC | 20 | 0.004 | 515 | 25.75 |
 Others | 103 | 0.023 | 2765 | 26.84 |
Hexanucleotide | 419 | 0.094 | 13704 | 32.71 |
 AAAAAC | 42 | 0.009 | 1326 | 31.57 |
 ACCTGC | 29 | 0.007 | 870 | 30.00 |
 AAGGTG | 23 | 0.005 | 690 | 30.00 |
 AGGAGT | 19 | 0.004 | 570 | 30.00 |
 Others | 306 | 0.069 | 10248 | 33.49 |
 Total | 445139 | 100 | 9411582 | 21.14 |