Generation of the surGasp1 transgenic mice. A) Schematic illustration of the surGasp1 transgene construct. To generate transgenic mice, a 3578 bp SalI-NsiI fragment comprising the Gasp1 coding sequence under the CMV promoter was injected into pronucleus of the one cell mouse embryo. S:SalI ; N:NsiI. B) Quantitative PCR analysis of Gasp1 expression on muscle (light grey) and brain (dark grey) samples from F1 mice of the six independent transgenic lines generated.