Figure 4

Diversity in virulence factor genes/loci among the classical Bordetellae compared to RB50. The heatmap was generated based on nucleotide percentage identity compared to RB50 for each gene/loci. Absence of a certain gene and presence of a pseudogene are highlighted with white and sky blue color with #, respectively. * indicates the missing nucleotides due to the draft status of the genome. The gene content tree, the dendrogram of hierarchical clustering of the complete pan-genome matrix, was superimposed on top of the heatmap. Manhattan distances, linkage method, and 1,000 bootstrap replicates were used for the clustering.