Figure 2
Predicted secondary structure of a. Alk-Exo, b. Helicase-2 and c. intervening polypeptide. Prediction was carried out on individual polypeptides and on the fusion protein in three elementary motifs: red cylinders represent α-helix, yellow arrows represent β-sheets, and green lines represent loop regions. Panel c. shows the predominance of undefined structure in the intervening polypeptide as well as in the connecting regions between the intervening polypeptide and the C-terminus of Alk-Exo and the N-terminus of helicase-2, respectively.