Table 3 miRNAs with predicted influence on SERCA2b expression
MicroRNA | ΔG binding (Kcal mol-1) | ΔG 70 nt flanking 3’ of predicted binding site (Kcal mol-1) | ΔG 70 nt flanking 5’ of predicted binding site (Kcal mol-1) | Predicted binding position in 3’-UTR SERCA2b | Perfect SERCA2b complimentarity to “seed” region |
|---|---|---|---|---|---|
miR-122 | −24.9 | −9.9 | −8.7 | 313-334 | no |
−23.7 | −3.0 | −8.2 | 788-809 | no | |
−21.5 | −0.2 | −9.5 | 496-517 | no | |
−19.3 | −8.2 | −7.5 | 253-274 | no | |
−19.3 | −3.5 | −5.2 | 476-497 | no | |
−19.3 | −6.3 | −9.0 | 458-479 | no | |
−18.1 | −4.3 | −8.3 | 151-172 | no | |
miR-199a-3p | −22.0 | −4.9 | −9.5 | 380-401 | yes |
−21.0 | −3.1 | −11.1 | 487-508 | yes | |
−18.6 | −8.3 | −3.9 | 59-80 | yes | |
−18.1 | −7.5 | −8.3 | 251-272 | no | |
miR-320a | −21.2 | −9.9 | −9.3 | 405-426 | no |
−21.8 | −9.0 | 0.3 | 798-819 | no | |
−21.2 | −4.7 | −9.8 | 65-86 | partially (1–4) | |
−23.7 | −8.2 | −8.2 | 687-708 | no | |
−21.8 | −9.1 | −11.9 | 123-144 | partially (1–6) | |
miR-320b | −21.2 | −9.9 | −9.3 | 405-426 | no |
−21.8 | −9.0 | 0.3 | 798-819 | no | |
−21.2 | −4.7 | −9.8 | 65-86 | partially (1–4) | |
−23.7 | −8.2 | −8.2 | 687-708 | no | |
−21.8 | −9.1 | −11.9 | 123-144 | partially (1–6) | |
miR-320c | −19.1 | −8.0 | 0.3 | 800-819 | no |
−24.0 | −12.4 | −8.2 | 689-708 | no | |
−21.1 | −11.4 | −9.2 | 650-669 | no | |
miR-320d | −23.4 | −13.2 | −8.2 | 690-708 | no |
−24.3 | −12.2 | −9.0 | 709-727 | partially (1–4, 6–7) | |
miR-483-5p | −23.8 | −13.1 | −9.0 | 705-726 | no |
−21.8 | −4.7 | −8.5 | 63-84 | no | |
−22.7 | −9.1 | −9.3 | 121-142 | no | |
−20.3 | −8.2 | −3.0 | 789-810 | Partially (1, 3–6, 8) | |
miR-574-3p | −22.9 | −8.2 | −6.5 | 347-368 | No |
−21.2 | −2.7 | −11.3 | 96-117 | no | |
miR-574-5p | −21.7 | −8.6 | −8.0 | 263-285 | No |
−23.4 | −4.9 | −3.5 | 479-501 | No | |
−18.2 | −3.3 | −3.5 | 12-34 | No | |
−20.5 | −9.0 | 0.3 | 797-819 | Partially (1–5, 7) | |
miR-140-3p | −19.9 | −8.2 | −6.5 | 348-368 | yes |
−20.5 | −9.9 | −3.5 | 484-504 | No | |
−19.7 | −8.7 | 0.3 | 799-819 | no |