Effects of immunostimulation on social behavior, chemical communication and genome-wide gene expression in honey bee workers (Apis mellifera)

Table 6 Primer sequences used to quantify expression of candidate genes from a third biological replicate using quantitative real-time PCR

Gene name	Primer direction	Sequence	Accession number/ reference
Lipid Storage Droplet 2	Forward	AATCGTGCTCG CAAAATACC	XM_003249187.1
Lipid Storage Droplet 2	Reverse	TTCGCAATAGGT AATGATTTTTCA	XM_003249187.1
Bubblegum	Forward	TTTTGCAAATCT TGGTGCAA	XM_624222.3
Bubblegum	Reverse	TTCTTCTGGTTT GCCTTCGT	XM_624222.3
Pale	Forward	TACTTCGTCGCG GATAGCTT	NM_001011633.1
Pale	Reverse	TACGCGAACGAT GTCTTCAG	NM_001011633.1
Groucho	Forward	ACTGTGGACAGG AGGATTGG	XM_392425.3
Groucho	Reverse	ATGAAGGACCTC GACGTTTG	XM_392425.3
Draper	Forward	ATCCTGCCACTG GTTATTGC	XM_624852.2.
Draper	Reverse	AGTCAACTCCCT CCCAACCT	XM_624852.2.
Apterous	Forward	AGGAAGAGGAAA CCCAAGGA	XM_392622.4
Apterous	Reverse	TCTGCGAGAGTTG CTTCAGA	XM_392622.4
Pebbled	Forward	CGCTGATACGTCA CCTCAGA	XM_003249649.1
Pebbled	Reverse	GCGAACTTCTCTC CACGTTC	XM_003249649.1
Defensin 1	Forward	TGCGCTGCTAACT GTCTCAG	(Evans,[2006])
Defensin 1	Reverse	CGAAACGTTTGTC CCAGAG	NM_001011616.2
Actin	Forward	CCTAGCACCATCC ACCATGAA	(Huising & Flik, 2005)
Actin	Reverse	GAAGCAAGAATTG ACCCACCAA	(Huising & Flik, 2005)

Primer sequences were designed in Primer3, v. 4.0 (http://frodo.wi.mit.edu/) or obtained from previous publications.

ISSN: 1471-2164