Figure 2

Biological processes affected by miR-181b over-expression in cell culture via miR-181b transfection. Panel A demonstrates the experimental design for the identification of genes subject to PTGS by increased miRNA concentrations. Canonical miRNA function results in a subsequent decrease in mRNA expression levels detected by whole-genome expression analysis using microarrays. These differentially expressed genes are subsequently utilised for DAVID pathways analysis and correlated against predicted miRNA targets. Panel B shows the increase in miR-181b expression levels in comparison to controls for HEK-293, HeLa and SH-SY5Y cell types. Panel C shows a clustered-by-gene heat map from whole genome expression microarray data from each cell model, with n=2 per condition. Panel D shows the significantly enriched KEGG pathways for each cell type in response to increased intracellular miR-181b levels. RI: receptor interaction; ECM: extracellular matrix; MAPK: mitogen-activated protein kinase.