Figure 8

Comparison of canonical (left) and non-canonical (right) miRNA-mRNA relationship. Panel A, scheme. Panel B contains charts of accuracy and false discovery rates associated with Targetscan’s prediction of observed changes in mRNA expression. Panel C, pie charts illustrating the distribution of miR-181b and E2F1 target genes predicted using different algorithms and parameters in multiple cell types. Signal-to-noise ratio is shown to increase for both canonical and non-canonical function as stringency increases from genes modulated by either miR-181b over-expression or inhibition across at least two cell types; to genes modulated by either miR-181b over-expression or inhibition across all three cell types; to genes modulated by both miR-181b over-expression and inhibition across at least two cell types. Panel D contains charts of enriched KEGG pathways from genes modulated by either miR-181b over-expression or inhibition across at least two cell types. RI: receptor interaction. MAPK: mitogen-activated protein kinase.