Global pattern of H3K9me2 in human pluripotent and differentiated cells. A) A representative region that shows LOCKs in differentiated cells (HA, HAEC and HPF) but not PSCs (H1 and ADA-38). Shown are H3K9me2 signals of ChIP-chip experiments in a ~500 kb long region containing WSCD2 gene. Positive and negative log2 (ChIP/Input) rations are shown in blue and red, respectively. B) qPCR validation of ChIP-chip data. By comparing four cell lines in 23 regions (Figure S1), enrichments (log2 ChIP/Input) measured by ChIP-chip (Y axis) and ChIP-qPCR (X axis) are highly correlated (R2 = 0.87).