Identifying breast cancer risk loci by global differential allele-specific expression (DASE) analysis in mammary epithelial transcriptome

Table 2 Validation of DASE Candidate by Sanger Sequencing

Gene ID	SNP ID	Heterozygous samples	DASE events* by array	DASE events by sequencing
ACPP	rs14192	5	4	3
CHRAC1	rs10216653	6	5	1
DMBT1	rs11523871	6	6	6
MMP20	rs1784423	6	6	6
MUC16	rs2547068	6	4	3
PTTG2	rs6811863	5	5	4
SLAMF1	rs1061217	6	6	2^†
USP6	rs11658877	6	6	2^†
ZNF331	rs8110350	5	5	4
Total:		51	47	31 ^§

*: A positive DASE event is defined when the ratio of ASE levels from one allele to another allele is either more that 2 or less that 0.5.
^†: Only two samples were able to be validated because of unsuccessful cDNA sequencing at this locus for some samples.
^§: DASE were confirmed by sequencing in total 31 out of 39 samples whose cDNA sequencings were successful.

ISSN: 1471-2164