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Table 2 Comparison of assembly of pepper Sanger ESTs versus assembly of IGA reads

From: De novo assembly of the pepper transcriptome (Capsicum annuum): a benchmark for in silico discovery of SNPs, SSRs and candidate genes

Statistics EST Assembly Transcriptome Assembly
Number of Unigenes 31,196 123,261
Total assembled nucleotides 21,665,127 135,019,787
Average GC content (%) 41 39
Longest Contig Length 3,989 19,089
Average Contig Length 694.5 1,095
Median Contig Length 651 697
N50 702 1,647
Number/Percent Contigs Size < 1 KB 27,248 78,433
   1–2 KB 3,634 27,436
   2–3 KB 288 10,616
   3–4 KB 26 3,955
   4–5 KB 0 1,559
   5–10 KB 0 1,184
   10–20 KB 0 78