Table 2 Summary of the identifications obtained for Phaedon cochleariae -derived proteins using both LC-MS/MS and LC-MS E analyses
Protein band | Protein identification | Mascot (LC-MS/MS) | PLGS (LC-MSE) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
nb | MW | Description | Accession | MW | Score | Peptides | Coverage | Score | Peptides | Coverage |
1 | 22.3 | ni | ||||||||
2 | 26.3 | GH11-1 | HE962209 | 23.9 | 155 | 2 | 15 | 2642 | 4 | 24.8 |
GH11-2 | HE962210 | 23.7 | 116 | 4 | 22 | 1687 | 13 | 24.8 | ||
3 | 28.6 | ni | ||||||||
4 | 35.6 | GH28-7 | HE962199 | 40.9 | 117 | 5 | 11 | 1425 | 5 | 11.8 |
3-hydroxyacyl-CoA dehydrogenase | Contig11024 | 36.1 | 191 | 3 | 10 | 835 | 9 | 14.3 | ||
5 | 41.1 | GH28-9 | HE962201 | 38.2 | 261 | 5 | 18 | 2188 | 16 | 28.1 |
GH28-6 | HE962198 | 40.7 | 144 | 3 | 10 | 1172 | 5 | 15.5 | ||
6 | 44.3 | ni | ||||||||
7 | 43.7 | GH28-1 | HE962193 | 38.8 | 185 | 5 | 14 | 6650 | 54 | 29.2 |
GH28-3 | HE962195 | 39.8 | 119 | 3 | 11 | - | - | - | ||
GH16-1 | Contig27637 | 42.7 | 197 | 5 | 13 | 1904 | 16 | 22.2 | ||
GH1-1 | Contig25268 | 33.2* | 269 | 6 | 22 | 3028 | 17 | 22.3 | ||
8 | 25.3 | ni | - | |||||||
9 | 25.6 | Cysteine proteinase | - | 20.9* | - | - | - | 6518 | 11 | 54.8 |
10 | 28.1 | GH45-4 | HE962204 | 25.6 | 76 | 1 | 6 | 5377 | 9 | 30.4 |
GH45-5 | HE962205 | 25.4 | 99 | 2 | 13 | 1183 | 5 | 29.2 | ||
11 | 30.1 | GH45-7 | HE962207 | 25.8 | - | - | - | 2314 | 12 | 9.9 |