Figure 3From: Detection of genetic association and functional polymorphisms of UGDH affecting milk production trait in Chinese Holstein cattleExpression analysis of all combinations of SNPs Ex 1–1, Ex 1–2 and Ex 11–1. (A) Illustration of eight haplotype constructs for UGDH. (B) Statistical analysis of real-time RT-PCR data for the eight plasmid constructs. 18sRNA was used to normalize expression of each construct. Compared with g-a-c, g-c-t, a-a-t, a-c-c, a-a-c, g-a-t, and a-c-t decrease the expression of UGDH, by 51.8%, 53.9%, 61.6%, 63%, 59.7% and 68.2%, respectively. (C) Representative Western blotting images for eight constructs of UGDH and β-Actin. (D) Statistical analysis of the protein level of eight constructs of UGDH normalized to β-Actin, which indicated a-a-t, a-c-c, a-a-c, g-c-c, and a-c-t similarly decreased UGDH protein expression, by 37.8%, 51.1%, 34.8%, 26.6%, and 50.1%, respectively, compared with the construct g-a-c. Values are given as means ± S.E.M. (* P <0.05, ** P <0.01; n = 5/group.Back to article page