Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote

Table 1 Summary of data generated for catfish transcriptome using Illumina Hiseq 2000 sequencing

Sequencing	No. of tissues*	No. of raw reads	Read length (bp)	No. of reads after trim	Avg. length after trim (bp)	No. of bases after trim (Gbp)
s_4_1_1	19	49,077,054	100	48,743,295	92.8	4.5
s_4_1	19	128,862,236	100	124,882,873	87.6	10.9
s_5_1	19	137,764,408	100	133,926,741	87.1	11.7
Total	-	315,703,698	100	307,552,909	88.0	27.1

*Sequencing was conducted using the total RNA isolated from nineteen tissues of a doubled haploid channel catfish. Tissues include head kidney, fin, pancreas, spleen, gill, brain, trunk kidney, adipose, liver, stomach, gall bladder, ovary, intestine, thymus, skin, eye, swim bladder, muscle, and heart.

ISSN: 1471-2164