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Table 6 Summary of full-length transcript identification after manual inspection

From: Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote

 

Number

Potential full-length transcripts

16,869

Full-length transcripts

14,240

Transcripts lack at 5' end

1,725

Transcripts lack at 3' end

711

Transcripts with incorrect ORFs

193

  1. A total of 16,869 potential full-length transcripts that matched 90% length of reference proteins were selected for manual inspection. Full-length transcripts were defined as transcripts contain a complete CDS if the ORF revealed a start codon and stop codon in agreement with the match in the database.