Molecular modeling and docking of CYP397A1V2 with substrate insecticides. (A) Cartoon representation of the homology model of CYP397A1V2. The cartoon model of CYP397A1V2 is colored using a blue to red gradient from N-terminus to C-terminus. The model was created with MODELLER 9v8 using human cytochrome P450 CYP3A4 as a template. (B) Clipped view of the 'V' shaped molecular surface of the active site cavity. (C-F) Insecticide substrates docked into the binding site of CYP397A1V2 represented as a cartoon model: DDT (C), imidacloprid (D), deltamethrin (E), and permethrin (F), depicted in a stick model (light green). CYP397A1V2 residues interacting with ligand rendered as the line model. The images were made using Pymol pymol.sourceforge.net.