Figure 1
From: Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery
Southern blot analysis of transformed poplar lines carrying the plasmids p6-HSP-TP-OCS and p7N-ATDs- rolC. A representative blot with Sca I restricted and nptII-probed DNA isolated from Agrobacterium strain used for transformation, negative control line Esch5, and eleven transgenic lines N82-2 to -5, N82-7 to -8, N82-10 to -12, N82-14 to -15 is shown. Sca I has only one restriction site in the cassette that can be used for copy number determination. Genomic DNA was separated on agarose gel, blotted and hybridized with a DIG-labelled nptII probe. A.t.: positive control (Agrobacterium tumefaciens), Esch5: non-transformed control line.